The Cryoplunge 3 is used to prepare frozen hydrated specimens for use in the JEOL 3200 FS. A small volume of the specimen is applied to the specimen grid, which is attached to plunging tweezers. The grid is loaded within the humidity chamber and gently blotted to produce a thin, aqueous film. The specimen grid is then plunged into a temperature controlled ethane bath. This results in instantaneous freezing of the specimen in a layer of non-crystalline (vitreous) ice. The EM grid is then kept at at liquid nitrogen temperatures and transfered from the forceps to a grid box. The cryo grid storage box can then be safely transferred to a liquid nitrogen storage vessel or to the workstation of the cryo transfer holder for low dose imaging on the TEM.